METHOD FOR USING TANGENTIAL FLOW ULTRAFILTRATION TECHNOLOGY TO PREPARE CONTROLLABLE HIGH CONCENTRATION SILK FIBROIN SOLUTION

The present invention proposes a method for preparing a controllable high-concentration silk fibroin solution with high molecular weight using tangential flow ultrafiltration technology. The method comprises: acquiring an inorganic salt solution of silk fibroin; subjecting the inorganic salt solution of silk fibroin to membrane clarification process; desalting the inorganic salt solution of silk fibroin by filtering the clarified solution obtained from the membrane clarification process through a tangential flow filtration system; and concentrating the silk fibroin solution to give a high-concentration aqueous solution of silk fibroin with high molecular weight. Compared with the prior art, the present invention achieves the desalination and concentration of a salt solution of silk fibroin by the tangential flow filtration technology, and directly acquires an aqueous solution of silk fibroin with a number-average molecular weight of 80 kDa-200 kDa. The obtained material has improved properties in all aspects. The method of the present invention greatly improves the efficiency and reduces the cost of producing silk fibroin solutions, and further more provides more convenience for the development of silk fibroin materials.