METHOD AND DETECTION KIT FOR THE DETECTION OF PYROGENS IN BIOLOGICAL SAMPLES

The invention relates to a method and a detection kit for the detection of pyrogens in a biological sample, in which immune cells, preferably the monocytic cell line THP-1 or THP-1 macrophages, are brought into contact with the sample, which may contain pyrogens, whereby the expression of immunomodu...

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Bibliographische Detailangaben
Hauptverfasser:	PALAND, Nicole, VOLLENBROICH, Dirk
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method and a detection kit for the detection of pyrogens in a biological sample, in which immune cells, preferably the monocytic cell line THP-1 or THP-1 macrophages, are brought into contact with the sample, which may contain pyrogens, whereby the expression of immunomodulatory mediators takes place, wherein after lysis of the immune cells the nucleic acids of the immune cells are extracted and the expression of immunomodulatory mediators is detected by means of PCR. At least one of three different cytokines/chemokines, preferably IL-1β, TNFa or CXCL8/IL-8, are detected in parallel. The invention can be used for the detection of endotoxin and non-endotoxin pyrogenic contaminations in a medicinal sample.