IN-VITRO SYNTHETIC PLATFORM FOR THE GENERATION OF ISOPRENOIDS

IN-VITRO SYNTHETIC PLATFORM FOR THE GENERATION OF ISOPRENOIDS

The invention relates to process for isoprenoid production by an in-vitro synthetic platform involving at least the steps of phosphorylating a first hemiterpene alcohol with a first phosphate donor under catalysis of a first kinase thereby obtaining a first hemiterpene monophosphate and phosphorylat...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DUDEK, Hanna, CZAJA, Rico, VOGEL, Andreas, BARTSCH, Sebastian, SCHILLING, Lena, DIRKMANN, Michael
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to process for isoprenoid production by an in-vitro synthetic platform involving at least the steps of phosphorylating a first hemiterpene alcohol with a first phosphate donor under catalysis of a first kinase thereby obtaining a first hemiterpene monophosphate and phosphorylating the latter with a second phosphate donor under catalysis of a second kinase thereby obtaining at least first hemiterpene diphosphate. The invention further relates to a composition comprising first hemiterpene diphosphate obtainable by the process according to the invention.