APPARATUS, SYSTEMS, AND METHODS FOR PREPARING AN OUTPUT SAMPLE WITH AERATION

Methods, devices, and systems for preparing an output sample of bacteria are disclosed. In one aspect, a method is disclosed comprising introducing an aliquot of a sample comprising the bacteria into a sample container such that the contained sample is in fluid communication with a reference sensor and an active sensor. The method also comprises incubating and aerating the contained sample at a flow rate of between 7.0 μL per second per mL of the contained sample and 10.0 μL per second per mL of the contained sample. The method further comprises monitoring a change in an ORP of the contained sample using a reader electrically coupled to the reference sensor and the active sensor and cooling the contained sample when a concentration of the bacteria in the contained sample is determined to have reached a desired or target concentration or within acceptable error margins thereof.