LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) ANALYSIS FOR PATHOGENIC TARGETS

The present disclosure is drawn to compositions and methods for loop-mediated isothermal amplification (LAMP) analysis utilizing a pH-dependent output signal. The composition can comprise a pH sensitive dye, and a plurality of non-interfering LAMP reagents. The method can comprise providing an assem...

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Bibliographische Detailangaben
Hauptverfasser:	SEVILLE, Jordan, MARUTHAMUTHU, Murali Kannan, DEXTRE, Andres, WANG, Jiangshan, VERMA, Mohit, MCCHESNEY, Darby
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure is drawn to compositions and methods for loop-mediated isothermal amplification (LAMP) analysis utilizing a pH-dependent output signal. The composition can comprise a pH sensitive dye, and a plurality of non-interfering LAMP reagents. The method can comprise providing an assembly of a solid phase medium and a composition, depositing a biological sample onto the solid phase medium, and heating the assembly to an isothermal temperature sufficient to facilitate a LAMP reaction.