METHODS AND SYSTEMS FOR DETERMINING KINETIC PARAMETERS

Methods and systems for determining kinetic parameters are provided. The methods and systems may use a surface having a matrix attached thereto and probe molecules bound to the matrix. Target molecules may be introduced and bind reversibly to the probe molecules. As target molecules are introduced, a first amount of intermolecular complex is generated between the target molecules and the probe molecules and monitored. Once a threshold first amount intermolecular complex is exceeded, introduction of the target molecules may cease. At this point, competitive inhibitor molecules may be introduced and bind to free target molecules produced from continued dissociation of the intermolecular target-probe complex. An amount of the first intermolecular complex may be monitored. This amount may be indicative of the kinetics of a second intermolecular complex between the free target molecules and the competitive inhibitor molecules. In this manner, kinetic parameters of the second intermolecular complex may be estimated.