NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD
The present invention provides a nucleic acid amplification method including:step A including annealing a Bw adapter primer comprising structure (a): 5'-B1c-BL-B1-N3c-3' containing an annealing region N3c, a loop region BL, and stem regions B1c and B1 and a target region of a target nuclei...
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| creator | ASANO Yoshinori NAKAZATO Hiroaki NOTOMI Tsugunori MORI Yuta |
| description | The present invention provides a nucleic acid amplification method including:step A including annealing a Bw adapter primer comprising structure (a): 5'-B1c-BL-B1-N3c-3' containing an annealing region N3c, a loop region BL, and stem regions B1c and B1 and a target region of a target nucleic acid, synthesizing a base sequence complementary to a base sequence on a 5'-end side of the target region starting from a 3'-end of the Bw adapter primer, and obtaining a first template nucleic acid in which a stem-loop structure is added to a 5'-end of a complementary strand of the target region;step B including annealing an Fw adapter nucleotide comprising structure (c): 5'-F1c-FL-F1-N5'-3' containing an annealing region N5', a loop region FL, and stem regions F1c and F1; and having an extension-inhibiting modification at the 3'-end and the first template nucleic acid, synthesizing a complementary strand of the Fw adapter nucleotide starting from a 3'-end of the first template nucleic acid, and obtaining a second template nucleic acid comprising structure (d): 5'-B1c-BL-B1-N3c-N5c-F1c-FLc-F1-3' in which a stem-loop structure is added to a 3'-end of the first template nucleic acid; andstep C including amplifying base sequence Nc comprising N3c and N5c of the second template nucleic acid by a LAMP method by using an Fw inner primer comprising structure (e): 5'-F1c-F2-3' and a Bw inner primer comprising structure (f): 5'-B1c-B2-3' with the second template nucleic acid as a template. |
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and having an extension-inhibiting modification at the 3'-end and the first template nucleic acid, synthesizing a complementary strand of the Fw adapter nucleotide starting from a 3'-end of the first template nucleic acid, and obtaining a second template nucleic acid comprising structure (d): 5'-B1c-BL-B1-N3c-N5c-F1c-FLc-F1-3' in which a stem-loop structure is added to a 3'-end of the first template nucleic acid; andstep C including amplifying base sequence Nc comprising N3c and N5c of the second template nucleic acid by a LAMP method by using an Fw inner primer comprising structure (e): 5'-F1c-F2-3' and a Bw inner primer comprising structure (f): 5'-B1c-B2-3' with the second template nucleic acid as a template.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241023&DB=EPODOC&CC=EP&NR=4269612A4$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76318</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20241023&DB=EPODOC&CC=EP&NR=4269612A4$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ASANO Yoshinori</creatorcontrib><creatorcontrib>NAKAZATO Hiroaki</creatorcontrib><creatorcontrib>NOTOMI Tsugunori</creatorcontrib><creatorcontrib>MORI Yuta</creatorcontrib><title>NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD</title><description>The present invention provides a nucleic acid amplification method including:step A including annealing a Bw adapter primer comprising structure (a): 5'-B1c-BL-B1-N3c-3' containing an annealing region N3c, a loop region BL, and stem regions B1c and B1 and a target region of a target nucleic acid, synthesizing a base sequence complementary to a base sequence on a 5'-end side of the target region starting from a 3'-end of the Bw adapter primer, and obtaining a first template nucleic acid in which a stem-loop structure is added to a 5'-end of a complementary strand of the target region;step B including annealing an Fw adapter nucleotide comprising structure (c): 5'-F1c-FL-F1-N5'-3' containing an annealing region N5', a loop region FL, and stem regions F1c and F1; and having an extension-inhibiting modification at the 3'-end and the first template nucleic acid, synthesizing a complementary strand of the Fw adapter nucleotide starting from a 3'-end of the first template nucleic acid, and obtaining a second template nucleic acid comprising structure (d): 5'-B1c-BL-B1-N3c-N5c-F1c-FLc-F1-3' in which a stem-loop structure is added to a 3'-end of the first template nucleic acid; andstep C including amplifying base sequence Nc comprising N3c and N5c of the second template nucleic acid by a LAMP method by using an Fw inner primer comprising structure (e): 5'-F1c-F2-3' and a Bw inner primer comprising structure (f): 5'-B1c-B2-3' with the second template nucleic acid as a template.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2024</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZEj2C3X2cfV0VnB09nRRcPQN8PF083R2DPH091PwdQ3x8HfRUQgI8vR1DVIIdg0Bsf2dXHUUHP1cFLw9QxTc_IMUCJrAw8CalphTnMoLpbkZFNxcQ5w9dFML8uNTiwsSk1PzUkviXQNMjMwszQyNHE2MiVACAEc_Mxc</recordid><startdate>20241023</startdate><enddate>20241023</enddate><creator>ASANO Yoshinori</creator><creator>NAKAZATO Hiroaki</creator><creator>NOTOMI Tsugunori</creator><creator>MORI Yuta</creator><scope>EVB</scope></search><sort><creationdate>20241023</creationdate><title>NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD</title><author>ASANO Yoshinori ; NAKAZATO Hiroaki ; NOTOMI Tsugunori ; MORI Yuta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_EP4269612A43</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; fre ; ger</language><creationdate>2024</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ASANO Yoshinori</creatorcontrib><creatorcontrib>NAKAZATO Hiroaki</creatorcontrib><creatorcontrib>NOTOMI Tsugunori</creatorcontrib><creatorcontrib>MORI Yuta</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ASANO Yoshinori</au><au>NAKAZATO Hiroaki</au><au>NOTOMI Tsugunori</au><au>MORI Yuta</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD</title><date>2024-10-23</date><risdate>2024</risdate><abstract>The present invention provides a nucleic acid amplification method including:step A including annealing a Bw adapter primer comprising structure (a): 5'-B1c-BL-B1-N3c-3' containing an annealing region N3c, a loop region BL, and stem regions B1c and B1 and a target region of a target nucleic acid, synthesizing a base sequence complementary to a base sequence on a 5'-end side of the target region starting from a 3'-end of the Bw adapter primer, and obtaining a first template nucleic acid in which a stem-loop structure is added to a 5'-end of a complementary strand of the target region;step B including annealing an Fw adapter nucleotide comprising structure (c): 5'-F1c-FL-F1-N5'-3' containing an annealing region N5', a loop region FL, and stem regions F1c and F1; and having an extension-inhibiting modification at the 3'-end and the first template nucleic acid, synthesizing a complementary strand of the Fw adapter nucleotide starting from a 3'-end of the first template nucleic acid, and obtaining a second template nucleic acid comprising structure (d): 5'-B1c-BL-B1-N3c-N5c-F1c-FLc-F1-3' in which a stem-loop structure is added to a 3'-end of the first template nucleic acid; andstep C including amplifying base sequence Nc comprising N3c and N5c of the second template nucleic acid by a LAMP method by using an Fw inner primer comprising structure (e): 5'-F1c-F2-3' and a Bw inner primer comprising structure (f): 5'-B1c-B2-3' with the second template nucleic acid as a template.</abstract><oa>free_for_read</oa></addata></record> |
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| language | eng ; fre ; ger |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD |
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