NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD

The present invention provides a nucleic acid amplification method including:step A including annealing a Bw adapter primer comprising structure (a): 5'-B1c-BL-B1-N3c-3' containing an annealing region N3c, a loop region BL, and stem regions B1c and B1 and a target region of a target nucleic acid, synthesizing a base sequence complementary to a base sequence on a 5'-end side of the target region starting from a 3'-end of the Bw adapter primer, and obtaining a first template nucleic acid in which a stem-loop structure is added to a 5'-end of a complementary strand of the target region;step B including annealing an Fw adapter nucleotide comprising structure (c): 5'-F1c-FL-F1-N5'-3' containing an annealing region N5', a loop region FL, and stem regions F1c and F1; and having an extension-inhibiting modification at the 3'-end and the first template nucleic acid, synthesizing a complementary strand of the Fw adapter nucleotide starting from a 3'-end of the first template nucleic acid, and obtaining a second template nucleic acid comprising structure (d): 5'-B1c-BL-B1-N3c-N5c-F1c-FLc-F1-3' in which a stem-loop structure is added to a 3'-end of the first template nucleic acid; andstep C including amplifying base sequence Nc comprising N3c and N5c of the second template nucleic acid by a LAMP method by using an Fw inner primer comprising structure (e): 5'-F1c-F2-3' and a Bw inner primer comprising structure (f): 5'-B1c-B2-3' with the second template nucleic acid as a template.