SYSTEMS AND METHODS FOR PRODUCING A BIOLOGIC MOLECULE WITH HYDROCYCLONE CELL RETENTION DEVICE

Systems and methods for continuous production of at least one biological molecule of interest are provided. More specifically, the systems include (i) at least one culture bioreactor; (ii) at least one hydrocyclone; and (iii) at least one production bioreactor; wherein the culture bioreactor and the production bioreactor are linked by the hydrocyclone. The methods include the steps of (i) culturing a plurality of host cells capable of producing a biologic molecule of interest in at least one culture bioreactor; (ii) inoculating at least one production bioreactor with cells obtained from step (i); and (iii) culturing the cells in the production bioreactor.