CELL CULTURE BAG, BIOREACTOR AND CELL FILTRATION DEVICE

A cell culture bag for cultivating cells under rocking conditions in a bioreactor system comprises a deformable flexible wall defining an expandable cell culture chamber for containing the cells in a cell culture medium. A filter is arranged inside the culture chamber dividing the culture chamber in a chamber top compartment for containing cell-free culture medium and a chamber bottom compartment for maintaining the cells. The filter comprises a membrane with a pore size configured to allow exchange of nutrients and gasses between the chamber bottom compartment and chamber top compartment while retaining cells in the chamber bottom compartment. The filter is connected to the deformable flexible wall of the cell culture bag via a filter support frame that is configured and arranged to support the filter and mitigate forces acting on the filter when filling and/or expanding and/or rocking the bag.