METHOD OF SEPARATING SINGLE STRANDED RNA MOLECULES FROM OTHER NUCLEIC ACID SPECIES, USE OF SOLID PHASE THEREFOR, AND METHOD OF SEPARATING DOUBLE STRANDED RNA MOLECULES BY SIZE

The present invention relates to a method of separating single-stranded RNA (ssRNA) molecules from other nucleic acid species and to the use of a solid phase for separating ssRNA molecules from other nucleic acid species. The invention claims that ssRNA could be eluted from a solid phase media compr...

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Bibliographische Detailangaben
Hauptverfasser:	Cernigoj, Urh, Miklavcic, Rok, Gagnon, Peter Stanley, Blaz, Bakalar, Dolenc, Darko
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method of separating single-stranded RNA (ssRNA) molecules from other nucleic acid species and to the use of a solid phase for separating ssRNA molecules from other nucleic acid species. The invention claims that ssRNA could be eluted from a solid phase media comprising multimodal ligands comprising nitrogen-containing heterocycles in an ascending pH gradient with: 1) a high product recovery at room temperature, 2) in the proximity of neutral pH, 3) without harmful additives.