A NON-HUMAN MAMMAL COMPRISING IN ITS GENOME AT LEAST TWO HUMAN LEUKOCYTE ANTIGEN (HLA) CLASS I ALLELES, METHODS OF MAKING SUCH MAMMAL AND USES THEREOF

The present invention relates to a non-human mammal comprising in its genome at least two human leukocyte antigen (HLA) class I alleles, wherein said at least two human HLA alleles are functionally expressed in that the corresponding MHC I polypeptides are expressed on the surface of cells of said mammal and present MHC antigens to which the non-human mammal provides an antigen-specific CD8+ T cell response and optionally wherein said at least two human HLA class I alleles comprise: and optionally wherein said at least two human HLA class I alleles comprise:a) at least one human HLA-A allele, and/orb) at least one human HLA-B allele, and/orc) at least one human HLA-C allele, and/ord) (a) and (b); and/ore) (a) and (c); and/orf) (b) and (c); and/org) (a), (b) and (c).The present invention further relates to uses of such a transgenic mammal for generating one or more T cell receptors that are capable of binding to an antigen of interest.