ENGINEERED CAS9-NUCLEASES AND METHOD OF USE THEREOF

Disclosed are SaCas9 protein variants, constructs encoding such variants, compositions comprising such variants and constructs, and methods of using the variants, constructs, and compositions. In some forms, the disclosed variants comprise the mutation Y239H and do not comprise the mutation R245A. A...

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Hauptverfasser:	YUEN, Tsz Lo, WONG, Siu Lun
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are SaCas9 protein variants, constructs encoding such variants, compositions comprising such variants and constructs, and methods of using the variants, constructs, and compositions. In some forms, the disclosed variants comprise the mutation Y239H and do not comprise the mutation R245A. Also disclosed are constructs encoding any of the disclosed variants for expression of the variant in a host of interest. Also disclosed are methods of editing a sequence of interest.