KITS, METHODS, POLYPEPTIDES, SYSTEMS, AND NON-TRANSITORY, MACHINE-READABLE STORAGE MEDIA FOR DETECTING A NUCLEIC ACID

Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucle...

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Hauptverfasser: ROLLER, Michael, HULL, Ian, KLINE, Enos, ATKINSON, Robert G, LUTZ, Barry R, WANG, Qin, LEON, Daniel, PANPRADIST, Nuttada
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creator ROLLER, Michael
HULL, Ian
KLINE, Enos
ATKINSON, Robert G
LUTZ, Barry R
WANG, Qin
LEON, Daniel
PANPRADIST, Nuttada
description Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.
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language eng ; fre ; ger
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title KITS, METHODS, POLYPEPTIDES, SYSTEMS, AND NON-TRANSITORY, MACHINE-READABLE STORAGE MEDIA FOR DETECTING A NUCLEIC ACID
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