KITS, METHODS, POLYPEPTIDES, SYSTEMS, AND NON-TRANSITORY, MACHINE-READABLE STORAGE MEDIA FOR DETECTING A NUCLEIC ACID

KITS, METHODS, POLYPEPTIDES, SYSTEMS, AND NON-TRANSITORY, MACHINE-READABLE STORAGE MEDIA FOR DETECTING A NUCLEIC ACID

Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucle...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ROLLER, Michael, HULL, Ian, KLINE, Enos, ATKINSON, Robert G, LUTZ, Barry R, WANG, Qin, LEON, Daniel, PANPRADIST, Nuttada
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Kits, methods, polypeptides, systems, and non-transitory, machine-readable storage media for detecting a nucleic acid in a sample are described. In an embodiment, the kit comprises a loop primer nucleic acid molecule configured for loop-mediated isothermal amplification (LAMP), the loop primer nucleic acid molecule comprising: a targeting sequence complementary to a target portion of a target nucleic acid sequence; and an adapter sequence; a displacement nucleic acid probe comprising: a fluorophore adapter sequence; and the adapter sequence; and a fluorophore adapter complement nucleic acid molecule complementary to the fluorophore adapter sequence, wherein the fluorophore adapter sequence or the fluorophore adapter complement nucleic acid molecule is coupled to a fluorophore. In an embodiment, the system comprises a thermal subsystem for heating a sample disposed therein, and an optical subsystem for optically excited the sample and detecting light emitted from the sample.