KIT OF GEL-FORMING SOLUTIONS INTENDED FOR PREPARATION OF HYDROGEL BASED ON COVALENTLY CROSSLINKED HYDROXYPHENYL DERIVATIVE OF HYALURONAN FOR PREVENTION OF POSTOPERATIVE COMPLICATIONS RELATED TO FORMATION OF COLORECTAL ANASTOMOSIS, USE OF KIT, METHOD OF PREPARATION OF HYDROGEL AND USE THEREOF

A kit of gel-forming solutions for a preparation of a biodegradable hydrogel based on a covalently crosslinked hydroxyphenyl derivative of hyaluronan is provided. The kit comprises at least two aqueous solutions, A and B, wherein the solution A comprises horseradish peroxidase and the solution B comprises hydrogen peroxide. At least one of the solution A and/or the solution B comprises a hydroxyphenyl derivative of a hyaluronan, and at least one of the solution A and/or the solution B comprises triclosan and hydroxypropyl-β-cyclodextrin. A method of preparing a hydrogel containing a covalently crosslinked hydroxyphenyl derivative of hyaluronan, from the kit, is also provided. The method comprises preparing separately the at least two solutions A and B, and mixing together the solution A with the solution B to form the hydrogel containing a covalently crosslinked hydroxyphenyl derivative of hyaluronan.