CELL CULTURE SUBSTRATE AND MANUFACTURING METHOD THEREOF

A cell culture substrate is provided. According to one embodiment of the present invention, the cell culture substrate includes a substrate provided with a non-porous surface and a cell culture coating layer that covers at least a portion of the non-porous surface, wherein the cell culture coating layer is implemented such that particles formed of fusion proteins for culturing cells are aggregated to form at least a portion of the surface, the fusion proteins comprising functional peptides bound to mussel adhesive proteins. In particular, despite containing compounds such as proteins that are beneficial to culturing cells, the cell culture substrate can be stored at room temperature for a long period of time over several years, exhibiting excellent storage stability, while activities of such compounds that are beneficial to culturing of the cells remain unchanged or only suffer minimal degradation so that the cells can be cultured at an initially-designed level. Further, the cell culture substrate has an excellent cell adhesion capability that allows reliable proliferation of the cells attached thereto, and thus can achieve a high cell culture efficiency. Accordingly, the cell culture substrate can be widely utilized for culturing various cells including stem cells.