METHOD AND KIT FOR DNA ISOLATION

A method for isolating cell-free DNA from liquid body sample, comprising the following steps: a) Providing liquid body sample; b) Adding to said sample: a solid phase capable of binding DNA; a binding buffer comprising a detergent and a chaotropic agent; and 2-propanol, to form a binding mixture the...

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Bibliographische Detailangaben
Hauptverfasser:	WEST, Richard Martin, DAVIES, Angela Sian, HATCHER, Malcolm John, SEIDEL, Monika
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for isolating cell-free DNA from liquid body sample, comprising the following steps: a) Providing liquid body sample; b) Adding to said sample: a solid phase capable of binding DNA; a binding buffer comprising a detergent and a chaotropic agent; and 2-propanol, to form a binding mixture thereof; c) Washing the solid phase to remove unbound material; and d) Eluting bound cell-free DNA, wherein the majority of the eluted DNA is