TUNABLE TRANSPOSON SYSTEMS

This invention provides methods and systems for enhancement of protein production from mammalian cell lines in a dmg inducible manner. The methods described herein can be used to generate a protein production cell line wherein the gene coding the protein product of interest is inserted into specific safe harbor loci (SHL) within the cell's genome and the gene copy number is induced to amplify by the use of an antibiotic inducer. The method enables for the conditional activation of the dmg inducible transposase. The dmg inducible gene amplification method described herein effectively functions as a molecular dial: combining dmg-inducible homologous recombination and conditional gene activation to fine-tune gene amplification in mammalian systems.