ELECTROCHEMICAL SENSING METHODS AND APPARATUS FOR DETERMINING DRUG UPTAKE AND RETENTION IN CELLS

This invention provides methods and apparatuses for the rapid assessment of cell permeability by a drug. More particularly, described herein a method of determining membrane permeability (influx and/or efflux) of a cell to a drug, the method including: (a) obtaining a biological sample; (b) dispersi...

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Bibliographische Detailangaben
Hauptverfasser:	KUSS, Sabine, LUU, Tran Le Huy
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBEMICROSCOPY [SPM] BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SCANNING-PROBE TECHNIQUES OR APPARATUS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention provides methods and apparatuses for the rapid assessment of cell permeability by a drug. More particularly, described herein a method of determining membrane permeability (influx and/or efflux) of a cell to a drug, the method including: (a) obtaining a biological sample; (b) dispersing at least one cell from the biological sample to a discrete location; (c) exposing the at least one cell to one member of a drug panel in a drug solution, wherein the drug panel is composed of drugs of a given concentration; (d) incubating the at least one cell from the biological sample in the drug for a given time; (e) obtaining at least one electro-analytical measurement of the discrete location adjacent the at least one cell.