TEMPLATE-FREE ENZYMATIC POLYNUCLEOTIDE SYNTHESIS USING DISMUTATIONLESS TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE VARIANTS

TEMPLATE-FREE ENZYMATIC POLYNUCLEOTIDE SYNTHESIS USING DISMUTATIONLESS TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE VARIANTS

The present invention is directed to the use of terminal deoxynucleotidyltransferase (TdT) variants lacking dismutation acivity for template-free enzymatic synthesis of polynucleotides of any predetermined sequence. Such TdT variants permit higher yields of correct sequence polynucleotides.

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Bibliographische Detailangaben
1. Verfasser: YBERT, Thomas
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention is directed to the use of terminal deoxynucleotidyltransferase (TdT) variants lacking dismutation acivity for template-free enzymatic synthesis of polynucleotides of any predetermined sequence. Such TdT variants permit higher yields of correct sequence polynucleotides.