METHOD FOR DETERMINATION OF AGGREGATES

The present disclosure provides a method for determining of aggregates comprising one or more macromolecules, in a first sample potentially comprising aggregates of the macromolecule(s), comprising the steps of (a) contacting a first sample with a sensing surface of an interaction analysis sensor, said sensing surface having immobilised thereon a ligand comprising a hydrophobic group, which is capable of increased binding interaction with aggregates of macromolecule(s) compared to non-aggregated macromolecule(s); (b) determining at least one parameter for the interaction of the first sample with the sensing surface; (c) Performing at least one of steps (i) and (ii): (i) Comparing the at least one parameter determined in step (b) with the corresponding parameter(s) determined for at least one additional sample potentially comprising aggregates of the macromolecule(s); (ii) determining at least one parameter related to aggregate(s) of the macromolecule(s); and (d) determining the presence, fraction, concentration, and/or amount of macromolecule(s) in the form of aggregate(s) in the first sample. The present disclosure also relates to uses of said method and an interaction analysis sensor for use in said method, as well as an interaction analysis sensor and a method for determining of the stability of a macromolecule.