ELECTROPORATION APPARATUS AND METHOD

Electroporation is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This allows for chemicals, drugs, and/or macromolecules such as proteins and nucleic acids to be introduced into the cells. Embodiments relate to an electropora...

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Bibliographische Detailangaben
Hauptverfasser: SCHMIDT, Scott, KRUGER, Kraig, INSLEE, Justin, REED, Thomas, D, GENTRY, Jeffrey, SCHNEIDER, Robert, GREEN, Chad B, BUTMAN, Bryan, CERRONE, David, CARSON, Jonathan, ANDREWS, Travis, MILLER, Mackenzie, SO, Vincent, ZHANG, Shuyuan, HOCK, William
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Electroporation is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This allows for chemicals, drugs, and/or macromolecules such as proteins and nucleic acids to be introduced into the cells. Embodiments relate to an electroporation apparatus. The electroporation apparatus comprises: a plurality of chambers configured to store a plurality of cells during an electroporation process; a plurality of electrodes configured to generate a plurality of electric fields within the plurality of chambers during the electroporation process, each electric field of the plurality of electric fields corresponding to one chamber of the plurality of chambers; a flow channel configured to transport the plurality cells during a cell collection process after the electroporation process; and a plurality of valves connecting the plurality of chambers to the flow channel.