PARTITION-BASED DETERMINATION OF TARGET COPY NUMBER FOR SINGLE CELLS BY NON-ENDPOINT AMPLIFICATION

Methods of analyzing a sample including cells and/or cell-free nuclei. In an exemplary method, partitions may be formed, with each partition including a portion of the same sample. Each partition of at least a subset of the partitions may contain only one of the cells/nuclei from the sample. Cells a...

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Hauptverfasser:	GREINER, Douglas, KARLIN-NEUMANN, George
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods of analyzing a sample including cells and/or cell-free nuclei. In an exemplary method, partitions may be formed, with each partition including a portion of the same sample. Each partition of at least a subset of the partitions may contain only one of the cells/nuclei from the sample. Cells and/or cell-free nuclei from the sample may be lysed in the partitions. At least one amplification reaction may be performed for a target or set of targets in the partitions. Amplification data may be collected from the partitions in an exponential/linear phase of each amplification reaction. A copy number of the target or set of targets may be determined for individual partitions using the amplification data, to determine if either a duplication or deletion is present in all or a subset of the cells analyzed.