NUCLEIC ACID MASS SPECTRUM NUMERICAL PROCESSING METHOD

A nucleic acid mass spectrum numerical processing method, comprising the following steps: step S1: re-calibrating a single mass spectrum graph; for each detection point of a sample, obtaining several mass spectrum graphs of different positions corresponding to the detection point, wherein each mass spectrum graph needs to be re-calibrated by using a special group of peaks, i.e. anchor peaks, that have an expected mass-to-charge ratio; step S2: synthesizing the mass spectrum graphs, and on the basis of step S1, synthesizing the several mass spectrum graphs of different positions corresponding to the detection point into a single mass spectrum graph of the detection point; step S3: performing wavelet filtering, and on the basis of step S2, eliminating high-frequency noise and a baseline by means of a wavelet-based digital filter; and step S4: extracting a peak characteristic value, and on the basis of step S3, performing peak fitting, and obtaining the peak height, peak width, peak area, mass offset, and signal-to-noise ratio on the basis of a fitting curve of the mass spectrum graph. According to the method, the reliability of nucleic acid mass spectrum data acquisition is improved, and the accuracy of nucleotide detection is improved.