DEVICE FOR THE SPECTROSCOPIC DETERMINATION OF THE BINDING KINETICS OF AN ANALYTE
The invention relates to a device for the label-free quantitative spectroscopic determination of the binding kinetics of an analyte. Essential components of the device, namely a light source (2), optical elements (5; 6; 7; 8; 9; 13; 13′) for beam guidance and for optically influencing the light of t...
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Zusammenfassung: | The invention relates to a device for the label-free quantitative spectroscopic determination of the binding kinetics of an analyte. Essential components of the device, namely a light source (2), optical elements (5; 6; 7; 8; 9; 13; 13′) for beam guidance and for optically influencing the light of the light source (2) and light modes emitted by a microsensor (functionalized spherical microparticle) retained in a microstructure (3) as a result of the exposure to the light of the light source (2), a spectrometer, which consists of an optical receiver (10) for the emitted light modes and an evaluation unit, actuators (14; 15) for positioning a carrier (4) with the microstructure (3) arranged thereon, and at least one control unit, are jointly arranged in an apparatus (1) having an apparatus housing (11). The light, namely the light of the light source (2) and the light modes emitted by a microparticle in question as a result of the exposure to said light, is guided in three different planes within the apparatus housing (11) by means of the optical elements (5; 6; 7; 8; 9; 13; 13), in particular by means of a first optical deflecting element (6) and by means of a second optical deflecting element (7). |
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