CELL CULTURING DEVICE, CELL CULTURING METHOD, AND METHOD FOR PREPARING PRODUCT

There is provided a cell culture device including a culture vessel containing a cell suspension and a diaphragm pump for extracting the cell suspension from the culture vessel. In the cell culture device, a volume of a space defined by a diaphragm of the diaphragm pump and a plane F including a surface of an outer edge part of the diaphragm on a side far from an apex of the diaphragm is 1 cm3 or more and 20 cm3 or less, in a case where an angle formed by a straight line connecting a foot of a perpendicular line which is drawn from the apex of the diaphragm to the plane F to each point in the diaphragm and by the perpendicular line is denoted by an angle A at each point, a thickness H of the diaphragm is within a range of 0.5 mm or more and 1.5 mm or less at any point in a region where the angle A is from 0° to 75°, and in a case where a thickness of the diaphragm at the apex of the diaphragm is denoted by HT, the thickness H of the diaphragm satisfies a relationship of 1 ≤ HT/H ≤ 1.75 at any point in the region where the angle A is from 0° to 75°. There is also provided a method for culturing cells using the cell culture device and a method for producing a product.