METHOD AND PRODUCTS FOR PRODUCING FUNCTIONALISED SINGLE STRANDED OLIGONUCLEOTIDES
The present invention relates to functionalized single stranded oligonucleotides and in particular to a method for producing functionalized single stranded oligonucleotides comprising: (a) providing a circular DNA molecule comprising an oligonucleotide sequence bordered by cleavage domains; (b) perf...
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| creator | HOGBERG, Bjorn DUCANI, Cosimo |
| description | The present invention relates to functionalized single stranded oligonucleotides and in particular to a method for producing functionalized single stranded oligonucleotides comprising: (a) providing a circular DNA molecule comprising an oligonucleotide sequence bordered by cleavage domains; (b) performing a rolling circle amplification (RCA) reaction with the circular DNA molecule of (a) as a template and one or more functionalized nucleotides (dNTPs); and (c) enzymatically cleaving the product of the RCA reaction at the cleavage domains to release the single stranded functionalized oligonucleotides. |
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(b) performing a rolling circle amplification (RCA) reaction with the circular DNA molecule of (a) as a template and one or more functionalized nucleotides (dNTPs); and (c) enzymatically cleaving the product of the RCA reaction at the cleavage domains to release the single stranded functionalized oligonucleotides.</description><language>eng ; fre ; ger</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2024</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240327&DB=EPODOC&CC=EP&NR=3921440C0$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20240327&DB=EPODOC&CC=EP&NR=3921440C0$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HOGBERG, Bjorn</creatorcontrib><creatorcontrib>DUCANI, Cosimo</creatorcontrib><title>METHOD AND PRODUCTS FOR PRODUCING FUNCTIONALISED SINGLE STRANDED OLIGONUCLEOTIDES</title><description>The present invention relates to functionalized single stranded oligonucleotides and in particular to a method for producing functionalized single stranded oligonucleotides comprising: (a) providing a circular DNA molecule comprising an oligonucleotide sequence bordered by cleavage domains; 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | METHOD AND PRODUCTS FOR PRODUCING FUNCTIONALISED SINGLE STRANDED OLIGONUCLEOTIDES |
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