COMPOSITIONS COMPRISING AN ENDONUCLEASE AND METHODS FOR PURIFYING AN ENDONUCLEASE

Provided are compositions comprising an endonuclease and methods for purifying an endonuclease. One aspect of the invention provides a composition comprising at least 100 mg of an untagged endonuclease having an A260/A280 absorbance ratio of from about X to about 0.8, wherein X is less than 0.8. Ano...

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Bibliographische Detailangaben
Hauptverfasser: SCHMITKE, Jennifer Louise, LYNG, Robert John, CHAN, Carol
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:Provided are compositions comprising an endonuclease and methods for purifying an endonuclease. One aspect of the invention provides a composition comprising at least 100 mg of an untagged endonuclease having an A260/A280 absorbance ratio of from about X to about 0.8, wherein X is less than 0.8. Another aspect of the invention provides a composition, generated by contacting a composition comprising at least 100 mg of an untagged endonuclease having an A260/A280 absorbance ratio of from about X to about 0.8, wherein X is less than 0.8, with an endonuclease binding molecule, wherein the endonuclease and the endonuclease binding molecule form a protein effector.