POLYPEPTIDE HAVING PHOSPHOLIPASE C ACTIVITY AND USE THEREOF

Provided is a polypeptide having phospholipase C activity. The polypeptide has: 1) an amino acid sequence represented by SEQ ID No: 2, comprising amino acid substitutions occurred at one or more positions, wherein the one or more positions are selected from positions 6, 8, 10, 104, and 205 in the am...

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Bibliographische Detailangaben
Hauptverfasser:	NIU, Qiwen, XU, Zhengjun, XUAN, Yaoji
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES BEER BIOCHEMISTRY CANDLES CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DETERGENTS ENZYMOLOGY ESSENTIAL OILS FATTY ACIDS THEREFROM METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFUMES PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a polypeptide having phospholipase C activity. The polypeptide has: 1) an amino acid sequence represented by SEQ ID No: 2, comprising amino acid substitutions occurred at one or more positions, wherein the one or more positions are selected from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2 or any combination thereof; or 2) having at least 80% sequence identity with 1), and at least one of positions 6, 8, 10, 104 and 205 being different from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2. Provided are a nucleic acid molecule for encoding the polypeptide, a vector comprising the nucleic acid molecule, and a cell comprising the nucleic acid molecule or the vector. Provided are uses of the polypeptide, the nucleic acid molecule, the vector, and the cell.