OPTIMIZED PRODUCTION OF CBGA FROM OLIVETOL ACID AND GERANYL PYROPHOSPHATE VIA SYNNPHB

The prenyltransferase is a protein which catalyzes the prenylation of olivetolic acid (OA) with geranyl pyrophosphate (GPP) to produce CBGA. However, CBGA production using prenyltransferase NphB is extremely poor and generates the side-product 2-O-geranyl olivetolate. This invention describes the pr...

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Hauptverfasser:	TABOADA, Andrea, SCHMITT, Patrick, DICK, Markus, SAITOV, Ali, MACIEJKO, Jakob
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The prenyltransferase is a protein which catalyzes the prenylation of olivetolic acid (OA) with geranyl pyrophosphate (GPP) to produce CBGA. However, CBGA production using prenyltransferase NphB is extremely poor and generates the side-product 2-O-geranyl olivetolate. This invention describes the prenyltransferase SynNphB from the Streptomyces sp. and a set of mutations which enhances the CBGA production by enhancing the turnover rate and specificity of the enzyme while decreasing the yield of the side-production 2-O-geranyl olivetolate in microorganisms.