IN-VITRO METHOD AND DEVICE FOR DETECTING A TARGET NUCLEID ACID

We disclose an in-vitro method for detecting a target nucleic acid in a patient's sample, comprising the following steps: Bringing the sample in contact with a probe that is a molecular beacon labeled with a fluorescent dye and which can hybridize with the target, then irradiating the probe wit...

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Bibliographische Detailangaben
1. Verfasser:	Fedorych, Oleh
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	We disclose an in-vitro method for detecting a target nucleic acid in a patient's sample, comprising the following steps: Bringing the sample in contact with a probe that is a molecular beacon labeled with a fluorescent dye and which can hybridize with the target, then irradiating the probe with light exciting the fluorescent dye, then observing the sample with a detector (15), and then, if the detector (15) detects light emitted by the probe, concluding that the target is in the sample.In order to speed up detection of the emitted light, the probe is immobilized in a carrier element (7) prior to bringing it in contact with the sample , wherein the carrier element (7) is in contact with an optical element (8) which lets the emitted light pass to the detector (15).