MANUFACTURING METHOD FOR SUBSTRATE ON WHICH NERVE CELLS ARE ARRANGED

An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells.A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrat...

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Bibliographische Detailangaben
Hauptverfasser:	MATSUMOTO, Takahiko, IWASHITA, Natsuko, YAMAZAKI, Takehiro, NONOYAMA, Yusuke, SAMESHIMA, Tatsuya, YAGINUMA, Hidekazu, YUMOTO, Masayuki, SATOH, Naoki
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	An object of the present invention is to provide a technique for precisely arranging nerve cells on a substrate while suppressing the migration of nerve cells.A manufacturing method for a substrate on which nerve cells are arranged is provided, the method including a step of arranging, on a substrate, a plurality of liquid droplets containing nerve cells by an inkjet method to form one or a plurality of liquid pools, the substrate having a region in which a cell adhesive material is arranged and a region in which a cell non-adhesive material is arranged; and a step of incubating the liquid pool until the nerve cells sediment and temporarily adhere onto the substrate to form a cell aggregate. The diameter per one liquid pool is 500 µm or less, and the density of nerve cells per one liquid pool is 105 cells/cm2 or more.