MULTI-SPECIFIC ANTIBODY, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

The present application provides a multispecific antibody and preparation method and use thereof. The multispecific antibody comprises:a) a Fab fragment specifically binding to a first antigen, wherein the Fab fragment consists of a light chain and CH1 and a variable region of a heavy chain;b) a fir...

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Bibliographische Detailangaben
Hauptverfasser:	LI, Hongjie, PAN, Xiujie, GAO, Xin, QIAN, Niliang, WANG, Fuyu, LIU, Yunhui, YANG, Cuima, HU, Dongmei
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present application provides a multispecific antibody and preparation method and use thereof. The multispecific antibody comprises:a) a Fab fragment specifically binding to a first antigen, wherein the Fab fragment consists of a light chain and CH1 and a variable region of a heavy chain;b) a first peptide linker with the N-terminal end fused to the heavy chain;c) a second peptide linker with the N-terminal end fused to the light chain,wherein only one disulfide bond can be formed between the first and the second peptide linkers, and each of the first and the second peptide linkers is independently selected from the group consisting of a peptide linker comprising any of the sequences as set forth in SEQ ID NO.1-2, wherein X represents any amino acid other than Cys, or is absent.The antibody can be readily produced by recombinant expression and can simultaneously target two different antigens or different epitopes of the same antigen, or multiple epitopes of more different antigens.