PRESSURE INSENSITIVE MICROFLUIDIC CIRCUIT FOR DROPLET GENERATION AND USES THEREOF

The present invention provides a microfluidic circuit for generating uniform droplets despite fluctuations in pressure, and manufacturing methods and uses thereof. Said circuit comprises microfluidic channels for carrying a continuous phase and a dispersed phase. In one embodiment, the ratio of the flow resistance of the dispersed phase to that of the continuous phase is equal to the ratio of the flow rate of the continuous phase to that of the dispersed phase. In one embodiment, the present microfluidic circuit comprises two features to achieve the desired ratio of flow resistance and flow rate of the dispersed phase and continuous phase: (a) using a single pressure source which applies identical pressure to the inlets of the upstream channels carrying the two phases, and (b) the flow resistance of the dispersed phase and continuous phase is much higher than the flow resistance of the downstream channel so that the flow resistance of the downstream channel become negligible.