METHOD FOR ESTIMATING CELL COUNT, AND DEVICE FOR ESTIMATING CELL COUNT

[Object]To provide a method and device for estimating the number of cells using a non-invasive process.[Solution]To estimate concentration of a cellular metabolite contained in a culture medium where certain cells are cultured, using a simple method, etc., spectroscopy. The number of the certain cel...

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Bibliographische Detailangaben
Hauptverfasser:	SAKURAI, Tsubasa, TAIRA, Keisuke
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	[Object]To provide a method and device for estimating the number of cells using a non-invasive process.[Solution]To estimate concentration of a cellular metabolite contained in a culture medium where certain cells are cultured, using a simple method, etc., spectroscopy. The number of the certain cells is estimatable by applying previously obtained information regarding relationship between a cellular metabolite concentration and the number of the certain cells.In spectroscopy, the higher the cellular metabolite concentration, the more accurately the actual concentration of the cell consumed-substance can be estimated. Accordingly, the concentration of a cell-consumed substance, decreasing as cells are cultured, are estimated in the early to middle stages of culture, and the concentrations of a cell-produced substance, increasing as cells are cultured, are estimated in the middle to late stage of the culture. This enables estimation of the number of cells in the entire range from beginning to end of cell culture.