METHOD FOR PREPARING VANILLIN BY FERMENTATION WITH EUGENOL AS SUBSTRATE

METHOD FOR PREPARING VANILLIN BY FERMENTATION WITH EUGENOL AS SUBSTRATE

The present disclosure discloses a method for preparing vanillin by fermentation with eugenol as a substrate, preparing vanillin by a mixed fermentation of Penicillium simplicissimum OMK-68 and Bacillus sp. OMK-69, its potency reached 35g/L, the mass conversion rate of eugenol reaches 83.3%. Penicil...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zeng, Yarou, Deng, Zhimin, Xing, Chenguang, Zhao, Xijing, LIU, Gang, LAN, Huanyan, LIU, Wei
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present disclosure discloses a method for preparing vanillin by fermentation with eugenol as a substrate, preparing vanillin by a mixed fermentation of Penicillium simplicissimum OMK-68 and Bacillus sp. OMK-69, its potency reached 35g/L, the mass conversion rate of eugenol reaches 83.3%. Penicillium simplicissimum OMK-68 in the present disclosure can use eugenol as a substrate for fermentation to prepare coniferyl alcohol, and its fermentation effect and substrate utilization rate are far better than wild-type Penicillium simplicissimum. The Bacillus sp. OMK-69 in the present disclosure can use coniferyl alcohol as a substrate for fermentation to prepare vanillin, and its fermentation effect and substrate utilization rate are far better than wild-type Bacillus sp.