METHOD AND APPARATUS FOR HIGH THROUGHPUT HIGH EFFICIENCY TRANSFECTION OF CELLS

METHOD AND APPARATUS FOR HIGH THROUGHPUT HIGH EFFICIENCY TRANSFECTION OF CELLS

Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: TANDON, Vishal, LISSANDRELLO, Charles A, SWIERK, Patricia A, BALESTRINI, Jenna L, COPPETA, Jonathan R
Format: Patent
Sprache:eng ; fre ; ger
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Transfer of genetic and other materials to cells is conducted in a hands-free, automated, high throughput, continuous process. A system using a microfluidic hydrodynamic sheath flow configuration includes arrangements for pushing cells from side streams containing a cell culture medium to a central stream containing an electroporation buffer. Electroporation can be conducted in an assembly in which two or more microfluidic channels are provided in a parallel configuration and in which various layers can be stacked together to form a laminate type structure.