METHOD FOR RAPID ACCURATE DISPENSING, VISUALIZATION AND ANALYSIS OF SINGLE CELLS

A method for performing analysis on single cells of interest, the method comprising:a) labeling at least one cell in a cell suspension with a first detectable label and a second detectable label, where the first detectable label identifies single cells and the second detectable label identifies cell...

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Bibliographische Detailangaben
Hauptverfasser: Schaal, Thomas D, Espinoza, Patricio, Srinivasan, Maithreyan, Mir, Alain-Albert, Dunne, Jude, Nasarabadi, Shanavaz Loharasp D, Lin, Chun-Wah
Format: Patent
Sprache:eng ; fre ; ger
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Zusammenfassung:A method for performing analysis on single cells of interest, the method comprising:a) labeling at least one cell in a cell suspension with a first detectable label and a second detectable label, where the first detectable label identifies single cells and the second detectable label identifies cells of interest in the suspension;b) dispensing a dispense volume of the suspension into wells of a multi-well device using a liquid dispensing system;c) generating a dispense map based on signals from the first detectable label and the second detectable label; andd) providing instructions, based on the dispense map, to the liquid dispensing system to dispense a reagent into wells containing the single cells of interest.