METHOD OF OPERATING EWOD DEVICE WITH SENSING APPARATUS

A method of determining a characteristic of a first pair of droplets in an electrowetting on dielectric microfluidic device substantially free from the influences of interference, comprises forming a droplet interface bilayer between a first pair of droplets, a droplet of the first pair of droplets...

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Bibliographische Detailangaben
Hauptverfasser:	HADWEN, Benjamin James, BROWN, Christopher James
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of determining a characteristic of a first pair of droplets in an electrowetting on dielectric microfluidic device substantially free from the influences of interference, comprises forming a droplet interface bilayer between a first pair of droplets, a droplet of the first pair of droplets contacting a first sensing electrode and another droplet of the first pair of droplets contacting a first bias electrode. A droplet interface bilayer is formed between a second pair of droplets, a droplet of the second pair of droplets contacting a second sensing electrode and another droplet of the second pair of droplets contacting a second bias electrode. A nanopore is inserted into the droplet interface bilayer of the first pair of droplets, a first signal is measured between the first bias electrode and the first sensing electrode due to transport of a target species through the nanopore of the first pair of droplets and a first signal is measured between the second bias electrode and the second sensing electrode for the second pair of droplets. A difference between the first measured signal from the first pair of droplets and the first measured signal from the second pair of droplets is measured, and the difference between the first measured signal from the first pair of droplets and the first measured signal from the second pair of droplets is reported as being the characteristic of the first pair of droplets, substantially free from interference.