METHOD FOR QUANTIFYING TARGET NUCLEIC ACIDS

The present invention has an object to provide a simple means capable of simultaneously determining absolute amounts of two or more types of target nucleic acids in a sample.The present inventors provide a method for determining absolute amounts of two or more types of target nucleic acids in a sample,the method comprising the steps of: (a) mixing a sample with a known amount of a control nucleic acid; (b) co-amplifying all target nucleic acids and a specific nucleic acid group to be collectively amplified with the target nucleic acids in the sample and the control nucleic acid; (c) determining a total amount of all target nucleic acids and the specific nucleic acid group in the sample based on an indicator of a total amount of the amplified all target nucleic acids and the specific nucleic acid group and an indicator of an amount of the amplified control nucleic acid; and (d) calculating an absolute amount of each target nucleic acid in the sample based on an occupancy of each target nucleic acid in the total of all target nucleic acids and the specific nucleic acid group which is calculated from the indicator of the total amount of the amplified all target nucleic acids and the specific nucleic acid group and an indicator of an amount of the amplified target nucleic acid.