METHOD FOR THE VITRIFICATION OF HUMAN SEMEN AND PORTABLE KIT FOR THE APPLICATION OF SAID METHOD

This invention describes a method for human sperm vitrification, which includes providing liquefied human sperm; having a vitrification medium, where the vitrification medium comprises: (a) a sperm buffer, and (b) a cryoprotectant mixture including a permeable cryoprotectant and a non-permeable cryo...

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Bibliographische Detailangaben
Hauptverfasser:	OBRADORS CHERTA, Albert, SÁNCHEZ GUTIÉRREZ, Raúl Segundo, ALVAREZ GONZÁLEZ, Juan Jesús, OQUIÑENA MUGUERZA, Enrique, SCHULZ RUBILAR, Mabel Andrea
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PEST REPELLANTS OR ATTRACTANTS PHYSICS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF TESTING TRAPPING
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Beschreibung
Zusammenfassung:	This invention describes a method for human sperm vitrification, which includes providing liquefied human sperm; having a vitrification medium, where the vitrification medium comprises: (a) a sperm buffer, and (b) a cryoprotectant mixture including a permeable cryoprotectant and a non-permeable cryoprotectant; mixing the liquefied sperm with the vitrification medium; providing a sperm straw and loading such straw with 0.25 to 0.5 mL of the obtained mixture; sealing the straw with heat; and placing the straw vertically in a container with liquid nitrogen to vitrify the mixture. This invention also provides a portable kit, which makes it easy to implement the method. Such kit includes a vitrification medium, some straws, a container, usage instructions, among others.