ABSOLUTE QUANTIFICATION OF TARGET MOLECULES AT SINGLE-ENTITY RESOLUTION USING TANDEM BARCODING

The present invention relates to a new method of labelling any target molecules from a plurality of entities, preferably in high throughput regimes, i.e. allowing the analysis of several thousands of entities per run, while preserving the integrity of the single-entity information. This method is ba...

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Bibliographische Detailangaben
Hauptverfasser:	RYCKELYNCK, Michaël, CUBI PIQUE, Roger, BAUDREY, Stéphanie
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a new method of labelling any target molecules from a plurality of entities, preferably in high throughput regimes, i.e. allowing the analysis of several thousands of entities per run, while preserving the integrity of the single-entity information. This method is based on a tandem molecular barcoding in which all molecular targets are labelled with a first unique barcode which is different for each molecular target from an entity, and with a tag sequence coding the entity from which the molecular target originates. Once this tandem barcoding is performed, the absolute quantification of all molecular targets with a single-entity resolution may be carried out in a single run of next-generation sequencing. The present invention also relates to a method of quantifying one or several molecular targets from a plurality of entities with single-entity resolution, as well as a kit and the use of such kit to label a plurality of molecular targets from a plurality of entities according to the method of the invention.