METHOD TO EXTRACT CHROMATIN FROM FORMALIN FIXED, PARAFFIN EMBEDDED (FFPE) TISSUE

Methods of extracting chromatin from tissue, such as formalin fixed, paraffin embedded (FFPE) tissue, are provided. The methods are rapid, simple, and preserve the chromatin signature. The methods can include, for example, removal of the tissue from the paraffin, enzymatic digestion of the extracell...

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Bibliographische Detailangaben
Hauptverfasser:	QUIMBY, Austin Louis, DAYTON, Paul Alexander, PATTENDEN, Samantha Gail, DAVIS, Ian Jonathan
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods of extracting chromatin from tissue, such as formalin fixed, paraffin embedded (FFPE) tissue, are provided. The methods are rapid, simple, and preserve the chromatin signature. The methods can include, for example, removal of the tissue from the paraffin, enzymatic digestion of the extracellular matrix, and exposure to ultrasound energy, optionally in the presence of a cavitation enhancement agent, such as microbubbles, nanobubbles, and/or phase-change nanodroplets. The methods can also include a mechanical tissue dissociation step. The methods provide chromatin fragments that are free of enzyme bias from fragmentation by enzymes such as micrococcal nuclease (MNase) and that are of optimal size for further quantification and/or identification. Kits for extracting chromatin from tissue are also provided.