LASER OPTICS ASSEMBLY OF FLOW CYTOMETER

A flow cytometer is disclosed. It comprises a flow cell defining a flow direction; a collimation assembly including laser diode and a collimating lens configured to produce a laser beam along an axis; a positive cylindrical lens disposed on the axis configured to receive the laser beam from the coll...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	MCELWAIN, Spencer Franklin, MISENER, Garland Christian, PAPALE, Alan, MOON, Michael Ryan
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS PHYSICS TESTING
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Beschreibung
Zusammenfassung:	A flow cytometer is disclosed. It comprises a flow cell defining a flow direction; a collimation assembly including laser diode and a collimating lens configured to produce a laser beam along an axis; a positive cylindrical lens disposed on the axis configured to receive the laser beam from the collimation assembly; and a negative cylindrical lens disposed on the axis and configured to receive the laser beam from the positive cylindrical lens. A cylindrical objective lens is disposed on the axis and is configured to receive the laser beam from the negative cylindrical lens and project the laser beam onto the flow cell such that the laser beam incident on the flow cell defines a first beam waist 1/e2 diameter in a direction parallel to the flow direction of the flow cell of 6.7 µm to 9 µm and a second beam 1/e2 diameter in a direction perpendicular to the flow direction of the flow cell of 190 µm to 210 µm.