METHODS FOR DETECTING TARGET POLYNUCLEOTIDES

The present invention provides a thermocycling method for increasing the number of tandem repeats of a unit sequence that is 1 to 60 nucleotides long in a linear polynucleotide. The invention also provides a solid substrate with a surface, the surface having at least one linear probe polynucleotide...

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Bibliographische Detailangaben
Hauptverfasser:	LITTLE, Rachel, PIKE, Andrew, TUITE, Eimer, IJIRO, Kuniharu, WHITFIELD, Colette, LUNN, Samantha, MITOMO, Hideyuki, BAHRA, Mantej, HEDLEY, Joseph
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a thermocycling method for increasing the number of tandem repeats of a unit sequence that is 1 to 60 nucleotides long in a linear polynucleotide. The invention also provides a solid substrate with a surface, the surface having at least one linear probe polynucleotide immobilised thereon, wherein the at least one linear probe polynucleotide comprises at least two tandem repeats of a unit sequence that is 1 to 60 nucleotides long. Methods of determining the presence of a linear target polynucleotide sequence in a test sample using such solid substrates are also provided.