BICISTRONIC EXPRESSION VECTOR FOR ANTIBODY EXPRESSION AND METHOD FOR PRODUCING ANTIBODY USING SAME

The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including &...

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Bibliographische Detailangaben
Hauptverfasser:	KIM, Soo Kwang, LEE, Yang Soon, SON, Yong Gyu, JUN, Hee Jung, KIM, Young Min, LEE, Dong Heon, CHOI, Yu Bin, AHN, Yong Ho, KIM, Eun A
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including 'promoter-UTR-intron-antibody light chain gene-polyA' and a second expression cassette including 'promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA'. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.