METHOD FOR INCREASING SECRETION OF RECOMBINANT PROTEIN

The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARD3 to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretio...

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Bibliographische Detailangaben
Hauptverfasser:	PARK, Jiyeon, BYUN, Hyunjong, AHN, Jung Hoon
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARD3 to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretion system (T1SS). The method can allows a protein be produced at a large amount simply and effectively without a separate purification process.