METHOD OF ISOLATING NUCLEIC ACID FROM SPECIMENS IN LIQUID-BASED CYTOLOGY PRESERVATIVES CONTAINING FORMALDEHYDE

There is described a method of processing a specimen that includes a clinical sample disposed in a liquid-based cytology preservative that comprises formaldehyde, the method comprising the steps of: (a) combining the specimen with proteinase K and formaldehyde scavenger to create a reaction mixture,...

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Bibliographische Detailangaben
Hauptverfasser:	WILSON, Timothy Joseph, JENSEN, Deborah Christine, KIRKCONNELL, Brett Wolfe
Format:	Patent
Sprache:	eng ; fre ; ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	There is described a method of processing a specimen that includes a clinical sample disposed in a liquid-based cytology preservative that comprises formaldehyde, the method comprising the steps of: (a) combining the specimen with proteinase K and formaldehyde scavenger to create a reaction mixture, wherein the formaldehyde scavenger is succinic acid dihydrazide; (b) incubating the reaction mixture at between 65 and 100 °C for a period of time sufficient to reverse chemical modifications of nucleic acid that may be contained in the specimen by formaldehyde in the liquid-based cytology preservative; (c) isolating a nucleic acid from the reaction mixture after the incubating step; and (d) performing anin vitroamplification reaction using the nucleic acid from the isolating step as templates.