METHOD AND APPARATUS FOR PERFORMING A NORMALIZATION IN THE CONTEXT OF SEQUENCING ANALYSIS

A method for performing a normalization of intensity values obtained to perform sequencing analysis comprises the steps: receiving (110) a plurality of image data for a plurality of channels, each image data (11a-11d, 15a-15d) describes for the respective channel (a-d) an intensity distribution over all positions of an image of the a plurality of image data (11 a-11 d, 15a-15d); parametrization (120) of the intensity distribution over all positions for the plurality of channels (a-d) to obtain parametrized distributions for the plurality of channels (a-d); combining (130) the parametrized distributions for the plurality of channels (a-d) to obtain a common distribution for all of the plurality of channels (a-d); and determining (140) for each of the plurality of channels (a-d) a transfer function such that the respective transfer function for the respective channel (a-d) maps the corresponding intensity distribution to the common distribution.